1ap rabbit anti ppt2 proteintech  (Proteintech)

Journal: EMBO Reports

Article Title: Palmitoylation by ZDHHC4 inhibits TRPV1-mediated nociception

doi: 10.1038/s44319-024-00317-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-PPT2 , Proteintech , Cat# 15429-1AP.

Techniques: Control, Recombinant, Plasmid Preparation, Sequencing, shRNA, Virus, Software, cDNA Synthesis, SYBR Green Assay

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: PPT2 is downregulated in ccRCC and has significant correlations with patients' survival. (A) Heat map depicting PPT family mRNA expression of all cases from TCGA database; red signifies high expression, yellow signifies medium expression, blue signifies low expression. (B) Comparison of PPT family mRNA expression in paired and in non-paired groups. (C-D) The mRNA expression of PPT2 and PPT1 was divided into low expression group and high expression group respectively on the basis of the median, the survival rate of two corresponding groups was evaluated with Kaplan Meier method and tested by using log rank test.

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Expressing, Comparison

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: Univariate and multivariate analyses for patient overall survival

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: Clinicopathological parameters and their correlations with PPT2 mRNA expression

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: The PPT2 expression may be considered as a diagnostic biomarker for ccRCC patients. The ROC curve analyses of PPT2 mRNA expression in subgroups of ccRCC patients show PPT2 expression is of significance in distinguishing ccRCC from normal renal tissues (A), M0 from M1 (B), G1+G2 from G3+G4 (C), T1+T2 from T3+T4 (D), TNM I+II from TNM III+IV (E), the recurred/progressed from the disease-free (F).

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Expressing, Diagnostic Assay, Biomarker Discovery

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: PPT2 expression is downregulated both in clinical ccRCC samples and in renal cancer cell lines. 32 pairs of postoperative clinical samples were collected, Western Blot (A), qRT-PCR (B) and IHC (C) were conducted in clinical ccRCC samples and in matched adjacent normal tissues. (D-E) Western Blot and qRT-PCR were also conducted to investigate PPT2 expression in cell lines 293, 786-O, A-498, ACHN, CAKi-1 and OS-RC.

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: Overexpression of PPT2 inhibits cell proliferation, migration and invasion in vitro . (A-B) The efficiency of PPT2 overexpression in cell lines A-498 and CAKi-1 transfected with expression vectors of PPT2 was verified with Western Blot and qRT-PCR, relative gene expression was determined using the comparative delta-delta CT method. (C) CCK-8 assays revealed cell growth curves of A498 and CAKi-1 cells. (D-E) Migration and invasion assays for ccRCC cell lines A498 and CAKi-1, representative photographs were taken at ×200 magnification; number of cells was counted in ten random images from each group. (F-G) Representative micrographs of wound healing assays of cell lines A-498 and CAKi-1, wound closures were photographed at 0, 12 and 24 hours after wounding, representative photographs were taken at ×100 magnification.

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Over Expression, Migration, In Vitro, Transfection, Expressing, Western Blot, Quantitative RT-PCR, Gene Expression, CCK-8 Assay

Journal: Journal of Cancer

Article Title: Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition

doi: 10.7150/jca.36477

Figure Lengend Snippet: Decreased expression of PPT2 facilitates EMT in ccRCC in vitro . (A-C) GSEA was performed to investigate the biological pathways involved in PPT2 regulation on the basis of TCGA database. (D-E) The alterations of EMT markers (E-cadherin, Vimentin, Snail1 and N-cadherin) were tested with Western Blot and qRT-PCR.

Article Snippet: Subsequently, after deparaffinization, rehydration, blocking with hydrogen peroxide and antigen retrieval, these sections were incubated with primary rabbit anti-PPT2 polyclonal antibodies (A12251, ABclonal; Wuhan, China) overnight at 4°C.

Techniques: Expressing, In Vitro, Western Blot, Quantitative RT-PCR